Efficacy of an experimental gaseous ozone-based sterilization method for clear aligners.

OBJECTIVES: To assess effectiveness of an experimental sterilization method based on the exposure of an O3/O2 gas mixture directly inside the packaging for clear aligners. MATERIALS AND METHODS: Fifty samples consisting of pieces of polyethylene terephthalate glycol (PET-G) aligners were contaminated by manual handling and subsequently divided into different groups (n = 30 for exposure to O3/O2 gas at different times, n = 10 for positive control with 2% chlorhexidine digluconate, n = 10 for negative control). The measurement of optical densities (OD) of the initial and final microbial cultures was recorded for all groups. Kruskal-Wallis test was used for differences between groups while Wilcoxon test was used to compare initial and final OD values within groups. Statistical significance was set at P < .05. RESULTS: Comparison within the groups showed statistically significant differences for exposure to the gaseous mixture (72 hours), for positive and negative controls. Other significant differences were found in the multiple comparisons between the application of gaseous ozone (48 hours and 72 hours) and the negative control. CONCLUSIONS: The direct exposure of gaseous ozone on the aligners inside their packaging showed microbicidal capacity at 72 hours, which was equivalent to the positive control with immersion in chlorhexidine digluconate. This innovative sterilization procedure could be considered in the final manufacturing processes of clear aligners to eliminate the potentially pathogenic microorganisms that are deposited on surfaces of these orthodontic devices.

A novel modified obturation technique using biosealers: An ex vivo study.

Background: The root canal anatomy is a complex space, and using a single-cone obturation technique does not guarantee a complete filling. Aim: This research aimed to evaluate the actual temperature of the biosealer during the obturation phase inside the root canal using a new hot technique. In addition, this study evaluated the penetration depth of the biosealer inside dentinal tubules. Setting and Design: The current study was concluded on two ex vivo experiments; the former tested the temperature evaluation. The latter examined the penetration depth of the biosealer. Materials and Methods: In this study, 42 sec mandibular premolars were used, and two experiments were carried out. In the first experiment, two premolars were utilized, and two thermocouples of K-type for each tooth were used. During the second experiment, the penetration depth of the biosealer was examined, 40 teeth were selected. Root canals were prepared then divided into two obturation groups: A, gutta-percha, and biosealer with single-cone technique and B, gutta-percha, and biosealer with a newly proposed obturation method. Confocal laser scanning was used to assess the penetration of the sealer. Statistical Analysis Used: In the first experiment, the Bonferroni method was performed to compare the temperature data. The tests used in the second experiment were Shapiro-Wilk's test, Kruskal-Wallis, and Mann-Whitney tests. Results: The first experiment results showed that the heat does not reach the apical third using the new obturation method. In the second experiment, the results showed in Group B significantly higher biosealer penetration into the dentinal tubules as compared to Group A (P < 0.05). Conclusions: The highest level of penetration of the biosealer in the dentinal tubules was observed in the group of the new obturation method, and the last apical 3 mm remained at 37° using this novel hot modified technique providing no risk of chemical alteration of the biosealer.

Evaluation of Mechanical Properties of a Hollow Endodontic Post by Three Point Test and SEM Analysis: A Pilot Study.

The aim of this study was to investigate the mechanical properties of a fiber hollow endodontic post characterized by the presence of an empty central cylindrical channel extended along the whole length. This particular shape allows clinicians to use the post also as a cementation resin carrier. Ten hollow posts were divided in two groups: the control group (unfilled hollow posts) (Group 0) and hollow posts filled with dual resin cement (Group 1). The samples of both groups were subjected to mechanical and micromorphological analysis by performing a three-point test and SEM observations. In the three-point test, the Group 1 samples exhibited a fracture load of 57.09 ± 5.06 N, a flexural strength of 1323.53 ± 110.09 MPa, and a Young's modulus of 42.87 ± 0.86 GPa. The samples of Group 2 exhibited a fracture load of 38.17 ± 1.7 N, a flexural strength of 908.87 ± 30.98 MPa, and a Young's modulus of 40.33 ± 1.9 GPa. The difference between fracture load, flexural strength, and deflection between the two groups was statistically highly significant (p < 0.01). Further, the difference between the Young's modulus of the two groups was statistically significant (p < 0.05). The values obtained are similar to those of other posts available on the market.